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Increased intake of dietary fat reduces insulin action in experimental animals, and this insulin resistance healingfats is associated with the accumulation of healingfats triglyceride in muscle and liver. In animal models, high-fat diets composed of fish oil or safflower oil have markedly different effects on insulin action, healingfats and these differences may depend on the ability of fatty acids in fish oil to upregulate lipid oxidation in the liver. Mechanisms may involve increased translocation and activation of specific protein kinase C isozymes (PKC- and PKC-) that phosphorylate and reduce the activity of insulin signaling intermediates.15 16 In C2C12 cells (a murine muscle cell line), oleate and palmitate have different effects on insulin-stimulated glucose conversion to glycogen and different effects on key components of the insulin signaling pathways.17 On the other hand, in humans, varying the total fat content of the diet does not affect insulin-mediated glucose disposal.18
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