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cells in vitro; (6-15) that the effects are associated with the production of lipid peroxides and aldehydes; (8-13) and that big plump plumpers galleries the cytotoxicity of the added PUFAs is reduced by the addition of antioxidants. (8-13) Studies with laboratory animals have also demonstrated that feeding a diet containing peroxidation products of fish oil (16) reduces tumor growth, and that the effect is reduced by administering antioxidants. (17,18) However, the effects in big plump plumpers galleries vitro are observed at PUFA concentrations (30 microM and above in most big plump plumpers galleries studies) exceeding normal plasma free fatty acid (FFA) levels. PUFAs in culture medium undergo lipid peroxidation more readily than those of plasma or tissues because: (1) culture medium, compared to plasma, contains lower levels of albumin that binds FFAs (19) and sequesters iron and copper that promote lipid peroxidation; (2) culture medium generally contains fewer antioxidants than plasma; (3) PUFAs in plasma lipoproteins are protected by antioxidants within the lipoproteins; and (4) cellular PUFAs are protected from lipid peroxidation by multiple antioxidants.
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