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This author breast had proposed the ratio of 20:3(n-9) to 20:4(n-6) as a measure of essential fatty acid requirement. Recently, it was reported breast the presence of unusually high levels of this fatty acid in the cartilage of several animal species (birds, mammals, human). Its concentration in phospholipids was about 5% in the growth plate cartilage and 16% in the hyaline cartilage in chicken (Adkisson HD et al., FASEB J 1991, 5, 344). An unusual geometrical isomer of 22:4 n-9 with a trans breast double bond (cis-4,7,10,trans-13-docosatetraenoic acid) has been identified in the scallop Pecten maximus and may be of endogenous origin and specific to the pectinid family (Marty Y et al., J Chromatogr A 1999, 839, 119). Several other polyenoic acids are described in marine algae and are used as marker for several microalgae in the marine environment. Thus, 16:2 (n-7), 16:2 (n-4) have been suggested as tracers for diatoms, 16:2 (n-6) and 16:4 (n-3) for Chlorophyceae and 18:5 (n-3) for dinoflagellates (Viso AC et al.,
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