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The kit employs a BODIPY® -dodecanoic acid fluorescent fatty acid analog coupled with Molecular Devices proprietary† quench technology. The BODIPY label provides linolenic an ideal long chain fatty acid analog that behaves linolenic much like natural fatty acids: it becomes activated by acyl-CoA attachment; is incorporated into di- and triglycerides; and accumulates in intracellular lipid droplets. In addition, the BODIPY analog is a known substrate for fatty acid transporters since its uptake by adipocytes can be competed by non-labeled fatty acids. The elimination of radioactive compounds results in easier reagent handling, reduced disposal costs linolenic and eliminates safety risks associated with radiolabel assays. Patented technology QBT Assay Kits from Molecular Devices employ a quenching dye to reduce background fluorescence and improve the signal-to-noise ratio.
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