Figure 3. Leptin inhibitory dress fatty fatty acid uptake
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Figure 3. Leptin inhibitory effect. 3T3L1 adipocytes were plated at 50,000 cells/well/100 µl of DMEM/FBS in 96-well plate for 5 hours, then serum deprived for 1 hour with or without leptin fatty acid uptake (100 nM). Insulin at 10 nM was then added into fatty acid uptake the well and incubated for a further 30 minutes at 37°C, 5% CO2 incubator. At the end of the incubation time, 100 µl of fatty acid was added into the well, and kinetic readings were started immediately with a FlexStation plate fatty acid uptake reader. Data courtesy of Cambridge Biotechnology. A: insulin, B: insulin and leptin, C: basal. Rapid assay development The streamlined homogeneous format makes the QBT Fatty Acid Uptake Assay Kit less labor intensive to run. With the single step mix-and-read procedure, the cells can be incubated with the reagents and are stable for up to several hours.
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