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Leatherhead Food fibromyalgia International uses GC analysis of fatty acid methyl esters (FAMES), which is more accurate. Where there are high levels of trans fatty acids present, it can be difficult to quantify the trans compounds because of interferences from cis fatty acids in the sample. In this case, silver-ion chromatography is used to separate the trans and cis isomers, and the trans isomers are fibromyalgia then re-analysed by GC. The combination of the data from the two GC analyses is then used to provide a value for the TFA content. Comparative studies of the two fibromyalgia analytical methods have shown that the GC based analysis generally provides the more accurate determination of the TFA content of a sample. General Comment Recommendations from a number of authoritative bodies have been published (for example UK Department of Health 1994, WHO 1994, BNF 1995, US FDA 2003, UK Food Standards Agency 2004). The consensus is that, although the risk to health of TFA intake at average consumption levels is small, the intake should not be increased.
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